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Image Search Results
Journal: bioRxiv
Article Title: Generation and Culture of Cardiac Microtissues in a Microfluidic Chip with a Reversible Open Top Enables Electrical Pacing, Dynamic Drug Dosing and Endothelial Cell Co-Culture
doi: 10.1101/2021.11.01.465885
Figure Lengend Snippet: Heart-on-chip features, configuration and fabrication process. A) Picture of the heart-on-chip device. B) on the left, the top schematic view of the heart on chip device depicting the monolithically integrated porous membrane in yellow; on the right, schematic cross-sectional view of the culture compartments (note: not to scale), the cardiac microtissues, its respective cell types and its real dimensions. C) General overview of the fabrication process of the heart-on-chip. i) Membranes are cut and fixed on the shapes mold using double sided tape; the mold parts were then brought together and the magnets applied in their respective pockets ensuring application of uniform pressure. ii) PDMS is degassed and injected into the mold using a syringe, bubbles are allowed to escape the mold and cured in an oven; iii) after PDMS curing, molds were separated and the heart-on-chip devices were diced after being peeled of the mold; iv) isometric schematic view of the assembled heart-on-chip device and the glass seal used to reversibly close the cardiac compartment and respective dimensions.
Article Snippet: To gain insight into the mechanism of drug diffusion between the different
Techniques: Membrane, Injection
Journal: bioRxiv
Article Title: Generation and Culture of Cardiac Microtissues in a Microfluidic Chip with a Reversible Open Top Enables Electrical Pacing, Dynamic Drug Dosing and Endothelial Cell Co-Culture
doi: 10.1101/2021.11.01.465885
Figure Lengend Snippet: Endothelialisation of the subjacent microfluidic channel. A) On the left, schematic top view of the HoC device and the dumbbell-shape cardiac compartment with the subjacent microfluidic channel. On the right, schematic cross-sectional view of the culture compartments and its respective cell types. B) Endothelialisation of the microfluidic channel in monoculture and co-culture conditions, both demonstrating good coverage of the microfluidic channel. C) Fluorescent microscopy micrograph of a closed-up view of the hPS-ECs cultured for up to 5 days on a rocking platform without any evident cell alignment. Flow direction was from left to right and vice-versa. In both B and C, the cytoskeleton of the hPS-ECs was stained with actin-green and their nuclei with DAPI in blue.
Article Snippet: To gain insight into the mechanism of drug diffusion between the different
Techniques: Co-Culture Assay, Microscopy, Cell Culture, Staining